▲ Echeveria laui tissue-cultured seedlings A succulent plant that has been steadily gaining popularity since it was introduced to Korea in the early 2000s.It has a cute appearance, is easy to grow, and has a fine dust purification function, so there is a lot of demand from consumers.In response, Gyeonggi Agricultural Research and Extension Services has recently succeeded in developing the technology by entering into a study on the mass propagation of rare succulents that are difficult to reproduce in collaboration with Mexico, the country of origin and largest possession of cacti and succulents.Gyeonggi Agricultural Research and Extension Services conducted a study called 'Establishment of a tissue culture system for the preservation of succulents resources and mass production' for three years from 2016 to this year in international collaboration with Autonoma University in Mexico.A succulent plant refers to a plant in which the stem or leaf of a plant has a thick fleshy texture with the development of a parenchymal tissue containing a lot of water, that is, a water reservoir.According to botanical taxonomy, there are more than 10,000 species, and there are more than 50 plants in the family Cactus, Liliaceae, and Crashulaceae.Among them, Echeveria is a plant native to Mexico, and about 400 species are in circulation.Although it is the most popular item among consumers, there are varieties that take a long time to generate new branches (new shoots) when breeding at farms, so a breeding technique method is required.▲ Echeveria elegans tissue-cultured seedling Hawolsia is a succulent plant that has recently been consumed domestically and internationally as a suitable indoor plant due to its strong sound tolerance (the property of a plant that performs photosynthesis and provides independent nutrition even in the dark).Most of Hawolsia is a rosette-shaped succulent plant (a leaf that is directly formed from the root and is attached to the ground), and the leaves are hard and have various colors.Hawolsia, which has a variety of species such as those with patterns on the upper side of leaves and those with transparent inner tissues, has a unique pattern and has high ornamental value, but is slow in growth and difficult to reproduce.In particular, Hawolsia 'Mansang' grows about 1 cm a year and takes more than 5 years from seed to adult, making it difficult for farmers to produce.To solve this problem, Gyeonggi Agricultural Research and Extension Services jointly developed tissue culture technology for mass production of Echeveria and Hawolsia species, which are difficult to reproduce, with Autonoma University in Mexico.▲ Research photo ■Succulent plant Echeveria mass propagation tissue culture technology Plant tissue culture refers to the use of plant typical performance (the ability to regenerate a complete plant from a part of plant tissue) to obtain a whole plant using a part of the plant under aseptic conditions. .In the Agricultural Research and Extension Services, Echeveria leaves are used in a medium to which plant hormones (plant growth materials) have been added (the role of supporting the cultured tissue and supplying nutrients). division and proliferation), and developed a technology that can propagate plants by forming new plant stems from callus.Among them, Echeveria laui contains 0.1 mg/L-1 of Naphthaleneacetic acid (NAA), an auxin, a plant hormone that helps root, and BA, a cytokine that is involved in cell division and plant differentiation (6- Benzylaminopurine) 3.0mg/L-1 mixed medium, 'Echeveria elegans', produced the most new shoots (new shoots) in NAA 0.3mg/L-1 single medium.As a medium suitable for mass propagation of the generated plant and formation of roots, 'Raui' is a mixture of NAA1mg/L-1 and BA1mg/L-1, and 'Elegance' is a mixture of NAA 1mg/L-1 and BA 0.1mg/L-1 Badges were selected.In addition, as a result of testing the appropriate cultivation environment to purify the sterile seedlings of Echeveria 'Raui' and 'Elegance' formed through this process, regardless of the type of soil, when acclimatized in 35-55% shade, it does not grow and is a complete plant. could getAs a result, in the case of Echeveria 'Raui' and 'Elegance', they were able to multiply 30 times and 80 times, respectively, compared to the general method of propagation using leaf shovels (a cultivation method in which leaves are cut and planted again to obtain plants).▲ Haworthia bunch tissue culture seedling ■ Succulent plant Haworthia mass propagation tissue culture technology Callus formation and shoot formation for mass propagation of Haworthia truncata and Haworthia maughanii, which are particularly slow growing among Haworthia plants. , developed tissue culture technology through the process of shoot propagation.Among them, a mixed medium of NAA 2mg·L-1 and TDZ 4mg·L-1 was selected as a medium suitable for the formation of 'Okseon' and 'Mansang' callus.From the generated callus, NAA 0.1mg/L-1 single medium was selected for 'Okseon' and 'Mansang' as suitable medium for Hawolsia shoot formation.In order to proliferate a large amount of plants and form roots, Haworthia 'Okseon' is NAA 1mg/L-1 and BA 0.1mg/L-1 medium, and Hawolsia 'Mansang' is NAA 0.1mg/L-1 and BA 0.1 It was found that the mg/L-1 mixed medium was effective.In addition, acclimatization of tissue culture sterile seedlings of Hawolsia 'Okseon' was able to obtain a complete plant without overgrowth when acclimatized in 95% shading in the same soil as Echeveria. It is now able to multiply by more than 1,000 times compared to the method of propagation usingThrough these results, it is expected that the establishment of tissue culture technology for rare succulents such as Echeveria and Hawolsia, which are difficult to reproduce, will lead the dissemination of technology for mass production of succulents and contribute to the industrialization of high value-added flowers.Kim Seok-cheol, director of Gyeonggi Agricultural Research and Extension Services, said, “We expect that the developed technology will contribute to the promotion of consumption of succulents and the creation of income for growers.Reporter Gu Ye-jiAmong them, Echeveria laui contains 0.1 mg/L-1 of Naphthaleneacetic acid (NAA), an auxin, a plant hormone that helps root, and BA, a cytokine that is involved in cell division and plant differentiation (6- Benzylaminopurine) 3.0mg/L-1 mixed medium, 'Echeveria elegans', produced the most new shoots (new shoots) in NAA 0.3mg/L-1 single medium.As a medium suitable for mass propagation of the generated plant and formation of roots, 'Raui' is a mixture of NAA1mg/L-1 and BA1mg/L-1, and 'Elegance' is a mixture of NAA 1mg/L-1 and BA 0.1mg/L-1 Badges were selected.Among them, a mixed medium of NAA 2mg·L-1 and TDZ 4mg·L-1 was selected as a medium suitable for the formation of 'Okseon' and 'Mansang' callus.In order to proliferate a large amount of plants and form roots, Haworthia 'Okseon' is NAA 1mg/L-1 and BA 0.1mg/L-1 medium, and Hawolsia 'Mansang' is NAA 0.1mg/L-1 and BA 0.1 It was found that the mg/L-1 mixed medium was effective.Suwon Headquarters: 6, Gyeongsu-daero 973beon-gil (Songjuk-dong), Jangan-gu, Suwon-si, Gyeonggi-do, 16303 Gyeonggi-do, Gyeonggi Daily B/D Tel: 031-250-3333 Incheon Headquarters: 21436 707 Gyeongin-ro, Bupyeong-gu, Incheon, Korea Tel: 032-439-2020The Gyeonggi Ilbo complies with the Internet Newspaper Commission's Code of Ethics.Copyright © 2022 Gyeonggi Daily.All rights reserved.